Ultra performance liquid chromatography (UPLC) takes advantage of small 1.7 µm particles operated at elevated pressures to achieve uncompromisable separation speed, resolution, and sensitivity (1-5). In order to tackle advantage of this new technology, it will be necessary to migrate methods from high performance liquid chromatography (HPLC) to UPLC, which can at first appear challenging. However, by selecting the proper column chemistry and dimensions, and properly scaling injection volume, flow rate and gradient conditions (if appropriate), the task of migrating methods is really quite easy and straightforward.
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