This is the best book for professional and students using HPLC. Basically, this book aims towards the solutions for the HPLC troubleshoots. The information what I got from this book is invaluable. At the bottom of the post you find the download link, remember it is for education purpose only.
The first manuscript of this textbook was written in 1977 for a training course in high-performance liquid chromatography (HPLC) for laboratory technicians at Berne. Its aim is to show the possibilities and problems associated with modern HPLC. The user of this challenging method needs a broad theoretical and practical knowledge and I hope that this book can impart both. To make things easier—and HPLC can be very easy—the theoretical background is restricted to the minimum.
Since the book is intended just to show the principles of HPLC, chapters dedicated to the separation of different classes of compounds have been omitted. The updates and improvements of this new edition are mainly to be found in details such as new references and technical descriptions which match today’s instrumentation. Four new sections have been written, namely on the shelf-life of mobile phases, the mixing cross, the phase systems in ion chromatography, and on measurement uncertainty. Some equations in the ‘zeroth chapter’, Important and Useful Equations for HPLC, have new numeric values because aporosity of 0.65 is more realistic than 0.8 for chemically bonded phases.
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